Reporter

Part:BBa_K2150028:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-13)


pTet+RBS+T7RNAP+Ter+pT7+RBS+tetX-GFP(fusion protein)+DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4703


Design Notes

Two different terminators are used to avoid repeated sequence.

Linker for fusion protein is carefully designed to avoid repeated sequence which may cause problems in assembly.


Source

T7RNAP:PCR amplified from Genome of BL21.

pT7:PCR amplified from BBa_K525998.

As for fusion protein tetX-GFP, tetX is the same as BBa_K2150101, the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).

References